CD45RA Monoklonaler Antikörper

CD45RA Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

HI100

Kat-Nr. : CL488-65108

Synonyme

B220, CD45, CD45R, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200

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Galerie der Validierungsdaten

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  FC experiment of human peripheral blood lymphocytes using CL488-65108

  1X10^6 human peripheral blood lymphocytes were surface stained with APC Anti-Human CD45RO (APC-65150, Clone: UCHL1) and 5.00 ul/test CoraLite® Plus 488-conjugated Anti-Human CD45RA (CL488-65108, Clone: HI100). Cells were not fixed.

• 

  FC experiment of human peripheral blood lymphocytes using CL488-65108

  1X10^6 human peripheral blood lymphocytes were surface stained with APC Anti-Human CD45RO (APC-65150, Clone: UCHL1) and CoraLite® Plus 488-conjugated mouse IgG2b isotype control antibody. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane Lymphozyten aus peripherem Blut

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-65108 bindet in FC CD45RA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: protein tyrosine phosphatase, receptor type, C
• GenBank-Zugangsnummer: BC014239
• Gene symbol: PTPRC
• Gene ID (NCBI): 5788
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei 2-8°C lagern. Vor Licht schützen.

Hintergrundinformationen

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).