CD45RA Rekombinanter Antikörper

CD45RA Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Ratte

Anwendung

FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

OX-33

Kat-Nr. : CL488-65578

Synonyme

CD45, Lca, Ptprc, L CA, OX-33

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  FC experiment of rat splenocytes using CL488-65578

  1x10^6 rat splenocytes were surface stained with APC Anti-Rat CD4 and 5 ul CoraLite® Plus 488 Anti-Rat CD45RA Mouse IgG2a Recombinant Antibody (CL488-65578, Clone: OX-33), or CoraLite® Plus 488 Mouse IgG2a Isotype Control (CL488-65208, Clone: C1.18.4). Cells were not fixed.

• 

  FC experiment of rat splenocytes using CL488-65578

  1x10^6 rat splenocytes were surface stained with 5 ul CoraLite® Plus 488 Anti-Rat CD45RA Mouse IgG2a Recombinant Antibody (CL488-65578, Clone: OX-33) (red), or CoraLite® Plus 488 Mouse IgG2a Isotype Control (CL488-65208, Clone: C1.18.4) (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Ratten-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-65578 bindet in FC CD45RA und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Leukocyte common antigen purified from rat splenocytes
• Vollständiger Name: protein tyrosine phosphatase, receptor type, C
• Berechnetes Molekulargewicht: 143 kDa
• GenBank-Zugangsnummer: XM_006249910
• Gene symbol: CD45
• Gene ID (NCBI): 24699
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signaling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).