CD45RA Rekombinanter Antikörper

CD45RA Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

PE-Cyanine7 Fluorescent Dye

CloneNo.

F8-11-13

Kat-Nr. : PY7-FcA65590

Synonyme

CD45, EC:3.1.3.48, GP180, L CA, LCA

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  FC experiment of human PBMCs using PY7-FcA65590

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 405 Anti-Human CD45RO (UCHL1), and 5 ul PE-Cyanine7 Anti-Human CD45RA (F8-11-13) Rabbit IgG RecAb (PY7-FcA65590, Clone: F8-11-13) or Rabbit IgG Isotype Control RecAb (PY7-FcA98136, Clone: 240953C9). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

PY7-FcA65590 bindet in FC CD45RA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: protein tyrosine phosphatase, receptor type, C
• Berechnetes Molekulargewicht: 1304 aa, 147 kDa
• GenBank-Zugangsnummer: BC014239
• Gene symbol: CD45
• Gene ID (NCBI): 5788
• Konjugation: PE-Cyanine7 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 450-500 nm / 778 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).