CD46 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CD46 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 12494-1-AP

Synonyme

CD46, MCP, Membrane cofactor protein, MIC10, TLX, TRA2.10

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Galerie der Validierungsdaten

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  WB analysis using 12494-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12494-1-AP (CD46 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 12494-1-AP

  IP Result of anti-CD46 (IP:12494-1-AP, 4ug; Detection:12494-1-AP 1:1000) with A549 cells lysate 1800ug.

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  IHC staining of human ovary tumor using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12494-1-AP (CD46 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12494-1-AP (CD46 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12494-1-AP (CD46 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12494-1-AP (CD46 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12494-1-AP (CD46 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12494-1-AP (CD46 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12494-1-AP (CD46 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12494-1-AP (CD46 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, Jurkat-Zellen, MOLT-4-Zellen
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:14000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• FC: See 1 publications below

Produktinformation

12494-1-AP bindet in WB, IP, IHC, FC, ELISA CD46 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD46 fusion protein Ag3131
• Vollständiger Name: CD46 molecule, complement regulatory protein
• Berechnetes Molekulargewicht: 384 aa, 43 kDa
• Beobachtetes Molekulargewicht: 50-56 kDa
• GenBank-Zugangsnummer: BC030594
• Gene symbol: CD46
• Gene ID (NCBI): 4179
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB, FC	Mol Immunol; Functional characterization of two novel non-synonymous alterations in CD46 and a Q950H change in factor H found in atypical hemolytic uremic syndrome patients.; Authors - Frida C Mohlin
human	WB	Oncol Rep; Bioinformatic analysis of the membrane cofactor protein CD46 and microRNA expression in hepatocellular carcinoma.; Authors - Zejun Lu ; KD Validated
mouse	WB	Clin Exp Immunol; Mitigating placental injuries through up-regulating DAF in experimental APS mice:new mechanism of progesterone.; Authors - Yingchun Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Andrea (Verified Customer) (04-12-2022)

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HeLa cells

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