CD46 Polyklonaler Antikörper

CD46 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28936-1-PBS

Synonyme

TRA2.10, TLX, MIC10, Membrane cofactor protein, MCP

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  WB analysis using 28936-1-AP (same clone as 28936-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 28936-1-AP (CD46 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28936-1-PBS in a different storage buffer formulation.

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  WB analysis using 28936-1-AP (same clone as 28936-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 28936-1-AP (CD46 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28936-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 28936-1-AP (same clone as 28936-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 28936-1-AP (CD46 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28936-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 28936-1-AP (same clone as 28936-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28936-1-AP (CD46 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28936-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 28936-1-AP (same clone as 28936-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28936-1-AP (CD46 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28936-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 28936-1-AP (same clone as 28936-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CD46 antibody (28936-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 28936-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

28936-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CD46 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD46 fusion protein Ag30376
• Vollständiger Name: CD46 molecule, complement regulatory protein
• Berechnetes Molekulargewicht: 384 aa, 43 kDa
• Beobachtetes Molekulargewicht: 50-70 kDa
• GenBank-Zugangsnummer: BC030594
• Gene symbol: CD46
• Gene ID (NCBI): 4179
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD46, also known as Membrane cofactor protein (MCP) or MIC10, is a ubiquitously expressed type type-I transmembrane glycoprotein that acts as a cofactor for complement factor I to mediate inactivation of C3b and C4b deposited on host cells. CD46 is a receptor for a large number of pathogens, including measles virus and adenovirus (PMID: 20941397). CD46 plays a role in linking innate and adaptive immune responses. It may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations of the CD46 gene have been associated with some diseases including hemolytic uremic syndrome (PMID: 26054645). CD46 is expressed on most cells as four isoforms that are derived via alternative splicing (PMID: 15324743).