CD47 Monoklonaler Antikörper

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• KD/KO Validated

CD47 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(19)

CloneNo.

1E1D8

Kat-Nr. : 66304-1-Ig

Synonyme

Leukocyte surface antigen CD47, Integrin-associated protein, Integrin associated protein, IAP, CD47 molecule

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  WB analysis using 66304-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 66304-1-Ig

  WB result of CD47 antibody (66304-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD47 transfected A549 cells.

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  WB analysis using 66304-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66304-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 66304-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CD47 antibody (66304-1-Ig, Clone: 1E1D8 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SKOV-3-Zellen, A549-Zellen, HaCaT-Zellen, HeLa-Zellen, HT-1080.Zellen, humanes Plazenta-Gewebe, HUVEC-Zellen, Jurkat-Zellen, K-562-Zellen, MDA-MB-231-Zellen, NCI-H1299-Zellen, TF-1-Zellen
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 17 publications below
• IF: See 3 publications below
• FC: See 1 publications below

Produktinformation

66304-1-Ig bindet in WB, IF/ICC, ELISA CD47 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD47 fusion protein Ag14132
• Vollständiger Name: CD47 molecule
• Berechnetes Molekulargewicht: 323 aa, 35 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC010016
• Gene symbol: CD47
• Gene ID (NCBI): 961
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.

Publikationen

Species	Application	Title
human	WB	Small; Bispecific Nanobody-Aptamer Conjugates for Enhanced Cancer Therapy in Solid Tumors; Authors - Ya Wang
human,rat	WB,IF	Redox Biol; Endothelial transferrin receptor 1 contributes to thrombogenesis through cascade ferroptosis; Authors - Haotian Ma
mouse	WB	ACS Appl Mater Interfaces; Dual-Regulated Biomimetic Nanocomposites For Promoted Tumor Photodynamic Immunotherapy; Authors - Li Liao
human,mouse	WB	J Control Release; Erythrocyte-biomimetic nanosystems to improve antitumor effects of paclitaxel on epithelial cancers.; Authors - Mingming Song
mouse	WB	J Nanobiotechnology; Bone-targeted erythrocyte-cancer hybrid membrane-camouflaged nanoparticles for enhancing photothermal and hypoxia-activated chemotherapy of bone invasion by OSCC.; Authors - Hongying Chen
human	WB	RSC Adv; Seedless preparation of Au nanorods by hydroquinone assistant and red blood cell membrane camouflage; Authors - Jing Li