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CD47 Monoklonaler Antikörper
CD47 Monoklonal Antikörper für WB, IF/ICC, ELISA
Wirt / Isotyp
Maus / IgG2b
Getestete Reaktivität
human und mehr (2)
Anwendung
WB, IF/ICC, ELISA
Konjugation
Unkonjugiert
CloneNo.
1E1D8
Kat-Nr. : 66304-1-Ig
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | SKOV-3-Zellen, A549-Zellen, HaCaT-Zellen, HeLa-Zellen, HT-1080.Zellen, humanes Plazenta-Gewebe, HUVEC-Zellen, Jurkat-Zellen, K-562-Zellen, MDA-MB-231-Zellen, NCI-H1299-Zellen, TF-1-Zellen |
| Erfolgreiche Detektion in IF/ICC | A549-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 17 publications below |
| IF | See 3 publications below |
| FC | See 1 publications below |
Produktinformation
66304-1-Ig bindet in WB, IF/ICC, ELISA CD47 und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| In Publikationen genannte Reaktivität | human, Maus, Ratte |
| Wirt / Isotyp | Maus / IgG2b |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | CD47 fusion protein Ag14132 |
| Vollständiger Name | CD47 molecule |
| Berechnetes Molekulargewicht | 323 aa, 35 kDa |
| Beobachtetes Molekulargewicht | 50-55 kDa |
| GenBank-Zugangsnummer | BC010016 |
| Gene symbol | CD47 |
| Gene ID (NCBI) | 961 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-A-Reinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for CD47 antibody 66304-1-Ig | Protokoll herunterladen |
| IF protocol for CD47 antibody 66304-1-Ig | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Redox Biol Endothelial transferrin receptor 1 contributes to thrombogenesis through cascade ferroptosis | ||
ACS Appl Mater Interfaces Dual-Regulated Biomimetic Nanocomposites For Promoted Tumor Photodynamic Immunotherapy | ||
J Control Release Erythrocyte-biomimetic nanosystems to improve antitumor effects of paclitaxel on epithelial cancers. | ||
J Nanobiotechnology Bone-targeted erythrocyte-cancer hybrid membrane-camouflaged nanoparticles for enhancing photothermal and hypoxia-activated chemotherapy of bone invasion by OSCC. | ||
RSC Adv Seedless preparation of Au nanorods by hydroquinone assistant and red blood cell membrane camouflage |




