CD47 Monoklonaler Antikörper

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• KD/KO Validated

CD47 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E1D8

Kat-Nr. : 66304-1-PBS

Synonyme

Leukocyte surface antigen CD47, Integrin-associated protein, Integrin associated protein, IAP, CD47 molecule

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  WB analysis of A549 using 66304-1-Ig (same clone as 66304-1-PBS)

  WB result of CD47 antibody (66304-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD47 transfected A549 cells. This data was developed using the same antibody clone with 66304-1-PBS in a different storage buffer formulation.

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  WB analysis using 66304-1-Ig (same clone as 66304-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66304-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66304-1-Ig (same clone as 66304-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66304-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66304-1-Ig (same clone as 66304-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66304-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 66304-1-Ig (same clone as 66304-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CD47 antibody (66304-1-Ig, Clone: 1E1D8 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66304-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66304-1-PBS bindet in WB, IF/ICC, Indirect ELISA CD47 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD47 fusion protein Ag14132
• Vollständiger Name: CD47 molecule
• Berechnetes Molekulargewicht: 323 aa, 35 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC010016
• Gene symbol: CD47
• Gene ID (NCBI): 961
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.