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CD47 Rekombinanter Antikörper

CD47 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242850C4

Kat-Nr. : 85958-1-PBS

Synonyme

IAP, Integrin-associated protein, Leukocyte surface antigen CD47

Filter:
  • Western Blot (WB) analysis of mouse spleen tissue using CD47 Recombinant antibody (85958-1-RR)

    WB analysis of mouse spleen using 85958-1-RR (same clone as 85958-1-PBS)

    Mouse spleen tissue was subjected to SDS PAGE followed by western blot with 85958-1-RR (CD47 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85958-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CD47 Recombinant antibody (85958-1-RR)

    IF Staining of RAW 264.7 using 85958-1-RR (same clone as 85958-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD47 antibody (85958-1-RR, Clone: 242850C4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85958-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

85958-1-PBS bindet in WB, IF/ICC, Indirect ELISA CD47 und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CD47 fusion protein Eg1394
Vollständiger Name CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
Berechnetes Molekulargewicht33 kDa
Beobachtetes Molekulargewicht45-55 kDa
GenBank-ZugangsnummerNP_001355344.1
Gene symbol Cd47
Gene ID (NCBI) 16423
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts as a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.