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CD47 Rekombinanter Antikörper

CD47 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242850C4

Kat-Nr. : 98456-3-PBS

Synonyme

IAP, Integrin-associated protein, Leukocyte surface antigen CD47

Filter:
  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse CD47 Rabbit Recombinant Antibody (98456-3-RR)

    FC experiment of mouse splenocytes using 98456-3-RR (same clone as 98456-3-PBS)

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD47 Rabbit RecAb (98456-3-RR, Clone:242850C4) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98456-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse CD47 Rabbit Recombinant Antibody (98456-3-RR)

    FC experiment of mouse splenocytes using 98456-3-RR (same clone as 98456-3-PBS)

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD47 Rabbit RecAb (98456-3-RR, Clone:242850C4) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98456-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98456-3-RR

    Affinity and Kinetic Characterization of 98456-3-RR

    Biolayer interferometry (BLl) kinetic assays of 98456-3-RR against Mouse CD47 were performed. The affinity constant is 0.273 nM.

Geprüfte Anwendungen

Produktinformation

98456-3-PBS bindet in FC CD47 und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CD47 fusion protein Eg1394
Vollständiger Name CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
Berechnetes Molekulargewicht33 kDa
GenBank-ZugangsnummerNP_001355344.1
Gene symbol Cd47
Gene ID (NCBI) 16423
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C.

Hintergrundinformationen

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts as a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.