CD47 Rekombinanter Antikörper

CD47 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242850C4

Kat-Nr. : 98456-3-RR

Synonyme

IAP, Integrin-associated protein, Leukocyte surface antigen CD47

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  FC experiment of mouse splenocytes using 98456-3-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD47 Rabbit RecAb (98456-3-RR, Clone:242850C4) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98456-3-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD47 Rabbit RecAb (98456-3-RR, Clone:242850C4) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98456-3-RR

  Biolayer interferometry (BLl) kinetic assays of 98456-3-RR against Mouse CD47 were performed. The affinity constant is 0.273 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98456-3-RR bindet in FC CD47 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD47 fusion protein Eg1394
• Vollständiger Name: CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
• Berechnetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: NP_001355344.1
• Gene symbol: Cd47
• Gene ID (NCBI): 16423
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts as a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.