CD48 Polyklonaler Antikörper

CD48 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31774-1-PBS

Synonyme

BLAST, BLAST 1, BLAST1, CD48 antigen, MEM 102

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  WB analysis using 31774-1-AP (same clone as 31774-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31774-1-AP (CD48/SLAMF2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31774-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 31774-1-AP (same clone as 31774-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31774-1-AP (CD48 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31774-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 31774-1-AP (same clone as 31774-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31774-1-AP (CD48 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31774-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

31774-1-PBS bindet in WB, IHC, Indirect ELISA CD48 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD48 fusion protein Eg1323
• Vollständiger Name: CD48 antigen
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 45-55 kDa
• GenBank-Zugangsnummer: BAE96326.1
• Gene symbol: Cd48
• Gene ID (NCBI): 12506
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Signaling lymphocytic activation molecule family 2 (SLAMF2, CD48) is an adhesion and costimulatory molecule expressed constitutively on most hematopoietic cells, particularly in antigen-presenting cells (APC). CD48 is expressed on all the hematopoietic cells, both in humans and mice, except for murine neutrophils and long-term HSC. CD48 exists as both a membrane-bound (mCD48) and a soluble (sCD48) form. CD48 can activate T cells, antigen-presenting cells and granulocytes, by binding to CD2 or bacterial FimH, and through cell intrinsic effects. Interactions between CD48 and its high-affinity ligand CD244 are more complex, with both stimulatory and inhibitory outcomes. CD48 expression is increased in autoimmunity and allergy diseases, and anti-CD48 monoclonal antibodies have been shown to attenuate experimental autoimmune encephalomyelitis.