CD48 Rekombinanter Antikörper

CD48 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

240732A5

Kat-Nr. : 98081-1-PBS

Synonyme

MEM 102, CD48 antigen, BLAST1, BLAST 1, BLAST

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  FC experiment of mouse splenocytes using 98081-1-RR (same clone as 98081-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.15 ug Anti-Mouse CD48 Rabbit Recombinant Antibody (98081-1-RR, Clone: 240732A5) or Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD19. Cells were not fixed. This data was developed using the same antibody clone with 98081-1-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98081-1-RR (same clone as 98081-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.15 ug Anti-Mouse CD48 Rabbit Recombinant Antibody (98081-1-RR, Clone:240732A5) (red) or Isotype Control (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98081-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98081-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98081-1-RR against Mouse CD48 were performed. The affinity constant is 1.48 nM.

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Geprüfte Anwendungen

Produktinformation

98081-1-PBS bindet in FC CD48 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: CD48 antigen
• Berechnetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BAE96326.1
• Gene symbol: Cd48
• Gene ID (NCBI): 12506
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

Signaling lymphocytic activation molecule family 2 (SLAMF2, CD48) is an adhesion and costimulatory molecule expressed constitutively on most hematopoietic cells, particularly in antigen-presenting cells (APC). CD48 is expressed on all the hematopoietic cells, both in humans and mice, except for murine neutrophils and long-term HSC. CD48 exists as both a membrane-bound (mCD48) and a soluble (sCD48) form. CD48 can activate T cells, antigen-presenting cells and granulocytes, by binding to CD2 or bacterial FimH, and through cell intrinsic effects. Interactions between CD48 and its high-affinity ligand CD244 are more complex, with both stimulatory and inhibitory outcomes. CD48 expression is increased in autoimmunity and allergy diseases, and anti-CD48 monoclonal antibodies have been shown to attenuate experimental autoimmune encephalomyelitis.