CD48 Rekombinanter Antikörper

CD48 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

240732A5

Kat-Nr. : 98081-1-RR

Synonyme

MEM 102, CD48 antigen, BLAST1, BLAST 1, BLAST

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  FC experiment of mouse splenocytes using 98081-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.15 ug Anti-Mouse CD48 Rabbit Recombinant Antibody (98081-1-RR, Clone: 240732A5) or Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD19. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98081-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.15 ug Anti-Mouse CD48 Rabbit Recombinant Antibody (98081-1-RR, Clone:240732A5) (red) or Isotype Control (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98081-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98081-1-RR against Mouse CD48 were performed. The affinity constant is 1.48 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98081-1-RR bindet in FC CD48 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: CD48 antigen
• Berechnetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BAE96326.1
• Gene symbol: Cd48
• Gene ID (NCBI): 12506
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

Signaling lymphocytic activation molecule family 2 (SLAMF2, CD48) is an adhesion and costimulatory molecule expressed constitutively on most hematopoietic cells, particularly in antigen-presenting cells (APC). CD48 is expressed on all the hematopoietic cells, both in humans and mice, except for murine neutrophils and long-term HSC. CD48 exists as both a membrane-bound (mCD48) and a soluble (sCD48) form. CD48 can activate T cells, antigen-presenting cells and granulocytes, by binding to CD2 or bacterial FimH, and through cell intrinsic effects. Interactions between CD48 and its high-affinity ligand CD244 are more complex, with both stimulatory and inhibitory outcomes. CD48 expression is increased in autoimmunity and allergy diseases, and anti-CD48 monoclonal antibodies have been shown to attenuate experimental autoimmune encephalomyelitis.