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CD48 Rekombinanter Antikörper

CD48 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240732E3

Kat-Nr. : 83871-1-PBS

Synonyme

MEM 102, CD48 antigen, BLAST1, BLAST 1, BLAST

Filter:
  • Western Blot (WB) analysis of various lysates using CD48 Recombinant antibody (83871-1-RR)

    WB analysis using 83871-1-RR (same clone as 83871-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83871-1-RR (CD48 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83871-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of EL-4-B5 cells using CD48 Recombinant antibody (83871-1-RR)

    IF Staining of EL-4-B5 using 83871-1-RR (same clone as 83871-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed EL-4-B5 cells using CD48 antibody (83871-1-RR, Clone: 240732E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83871-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83871-1-RR

    Affinity and Kinetic Characterization of 83871-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83871-1-RR against Mouse CD48 were performed. The affinity constant is 0.629 nM.

Geprüfte Anwendungen

Produktinformation

83871-1-PBS bindet in WB, IF/ICC, ELISA CD48 und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Rekombinantes Protein
Vollständiger Name CD48 antigen
Berechnetes Molekulargewicht27 kDa
Beobachtetes Molekulargewicht45-60 kDa
GenBank-ZugangsnummerBAE96326.1
Gene symbol Cd48
Gene ID (NCBI) 12506
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Signaling lymphocytic activation molecule family 2 (SLAMF2, CD48) is an adhesion and costimulatory molecule expressed constitutively on most hematopoietic cells, particularly in antigen-presenting cells (APC). CD48 is expressed on all the hematopoietic cells, both in humans and mice, except for murine neutrophils and long-term HSC. CD48 exists as both a membrane-bound (mCD48) and a soluble (sCD48) form. CD48 can activate T cells, antigen-presenting cells and granulocytes, by binding to CD2 or bacterial FimH, and through cell intrinsic effects. Interactions between CD48 and its high-affinity ligand CD244 are more complex, with both stimulatory and inhibitory outcomes. CD48 expression is increased in autoimmunity and allergy diseases, and anti-CD48 monoclonal antibodies have been shown to attenuate experimental autoimmune encephalomyelitis.