CD48 Rekombinanter Antikörper

CD48 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240732E3

Kat-Nr. : 83871-1-RR

Synonyme

MEM 102, CD48 antigen, BLAST1, BLAST 1, BLAST

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  WB analysis using 83871-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83871-1-RR (CD48 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of EL-4-B5 using 83871-1-RR

  Immunofluorescent analysis of (4% PFA) fixed EL-4-B5 cells using CD48 antibody (83871-1-RR, Clone: 240732E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83871-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83871-1-RR against Mouse CD48 were performed. The affinity constant is 0.629 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: CTLL-2 cells, Mausmilzgewebe
• Erfolgreiche Detektion in IF/ICC: EL-4-B5 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83871-1-RR bindet in WB, IF/ICC, ELISA CD48 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: CD48 antigen
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 45-60 kDa
• GenBank-Zugangsnummer: BAE96326.1
• Gene symbol: Cd48
• Gene ID (NCBI): 12506
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Signaling lymphocytic activation molecule family 2 (SLAMF2, CD48) is an adhesion and costimulatory molecule expressed constitutively on most hematopoietic cells, particularly in antigen-presenting cells (APC). CD48 is expressed on all the hematopoietic cells, both in humans and mice, except for murine neutrophils and long-term HSC. CD48 exists as both a membrane-bound (mCD48) and a soluble (sCD48) form. CD48 can activate T cells, antigen-presenting cells and granulocytes, by binding to CD2 or bacterial FimH, and through cell intrinsic effects. Interactions between CD48 and its high-affinity ligand CD244 are more complex, with both stimulatory and inhibitory outcomes. CD48 expression is increased in autoimmunity and allergy diseases, and anti-CD48 monoclonal antibodies have been shown to attenuate experimental autoimmune encephalomyelitis.