CD49b Monoklonaler Antikörper

CD49b Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Atlantic Blue™ Fluorescent Dye

CloneNo.

AK7

Kat-Nr. : AB-65252

Synonyme

BR, CD49B, Collagen receptor, GPIa, Integrin alpha 2, ITGA2, VLA 2, VLA 2 subunit alpha, VLAA2

---

• 

  FC experiment of human PBMCs using AB-65252

  1X10^6 human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human CD49b (AB-65252, Clone:AK7) or unstained. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using AB-65252

  1X10^6 human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human CD49b (AB-65252, Clone:AK7) or unstained. Cells were not fixed. Monocytes were gated.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

AB-65252 bindet in FC CD49b und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Humane Thrombozyten
• Vollständiger Name: integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
• Berechnetes Molekulargewicht: 126 kDa
• GenBank-Zugangsnummer: NM_002203
• Gene symbol: Integrin alpha 2
• Gene ID (NCBI): 3673
• Konjugation: Atlantic Blue™ Fluorescent Dye
• Excitation/Emission maxima wavelengths: 404 nm / 458 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD49b, also known as alpha2 integrin, is a type I transmembrane glycoprotein which forms an adhesion molecule VLA-2 with beta1 integrin. CD49b is a receptor for many (extracellular) matrix and non-matrix molecules (PMID: 23847623). CD49b is expressed on numerous cell types including platelets, activated T cells, B cells, and monocytes. CD49b activation skews NK cell effector function to a phenotype that exhibits elevated IFN-γ production with a concomitant inhibition of motility and cytolytic function (PMID: 32971468, 32762681). The Anti-Human CD49b antibody, clone AK7, is derived from Human platelets.