CD49b Monoklonaler Antikörper

CD49b Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgM, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

DX5

Kat-Nr. : CL647-65065

Synonyme

Itga2, integrin alpha 2, DX5

---

• 

  FC experiment of mouse splenocytes using CL647-65065

  1X10^6 mouse splenocytes were surface co-stained with FITC Anti-Mouse NK1.1 (CD161) (PK136) and 0.4 ug CoraLite® Plus 647-conjugated Anti-Mouse CD49b (CL647-65065, Clone: DX5) or 0.4 ug CoraLite® Plus 647-conjugated Rat IgM isotype control. Cells were not fixed.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65065 bindet in FC CD49b und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgM, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Aus C57BL/6-Mäusen isolierte NK-Zellen
• Vollständiger Name: integrin alpha 2
• GenBank-Zugangsnummer: BC065139
• Gene symbol: CD49b
• Gene ID (NCBI): 16398
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Integrins are a large family of α/β transmembrane receptors playing a key role in cell-cell interactions and cell adhesion to the extracellular matrix (PMID: 30374344). CD49b, also known as integrin alpha-2, is a 150 kDa transmembrane glycoprotein expressed on a wide variety of cell types including platelets, megakaryocytes, NK cells, NKT cells, neutrophils, and long-term activated T cells (PMID: 18802064; 2415516). Integrin alpha-2/beta-1 (also known as VLA-2) is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin (PMID: 2557634). It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and CLG gene expression, force generation and organization of newly synthesized extracellular matrix.