CD5 Polyklonaler Antikörper

CD5 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 17227-1-AP

Synonyme

CD5, CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, T1

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 17227-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17227-1-AP (CD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 17227-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17227-1-AP (CD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 17227-1-AP

  IP Result of anti-CD5 (IP:17227-1-AP, 4ug; Detection:17227-1-AP 1:500) with mouse liver tissue lysate 6800ug.

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  IHC staining of human tonsillitis using 17227-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17227-1-AP (CD5 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 17227-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17227-1-AP (CD5 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 17227-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD5 antibody (17227-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human tonsillitis using 17227-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD5 antibody (17227-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Mauslebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below

Produktinformation

17227-1-AP bindet in WB, IP, IHC, IF, ELISA CD5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD5 fusion protein Ag11065
• Vollständiger Name: CD5 molecule
• Berechnetes Molekulargewicht: 495 aa, 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa, 67 kDa
• GenBank-Zugangsnummer: BC027901
• Gene symbol: CD5
• Gene ID (NCBI): 921
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

Publikationen

Species	Application	Title
mouse	IHC	FASEB J; A self-organized actomyosin drives multiple intercellular junction disruption and directly promotes neutrophil recruitment in lipopolysaccharide-induced acute lung injury.; Authors - Bing Chen
human	IHC	Front Oncol; Whole-Exome Sequencing Reveals New Potential Mutations Genes for Primary Mucosa-Associated Lymphoid Tissue Lymphoma Arising From the Kidney.; Authors - Shuang Wen
human	WB	Hematol Oncol; Nuclear αvβ3 integrin expression, post translational modifications and regulation in hematological malignancies.; Authors - Avivit Weisz