CD5 Rekombinanter Antikörper

CD5 Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

L17F12

Kat-Nr. : 65599-1-PBS

Synonyme

CD5 molecule, L17F12, LEU1, Lymphocyte antigen T1/Leu 1, Lymphocyte antigen T1/Leu-1

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  FC experiment of human PBMCs using 65599-1-MR (same clone as 65599-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD5 Mouse IgG2a Recombinant Antibody (65599-1-MR, Clone: L17F12) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65599-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65599-1-MR (same clone as 65599-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD5 Mouse IgG2a Recombinant Antibody (65599-1-MR, Clone:L17F12) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65599-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65599-1-MR (same clone as 65599-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD5 Mouse IgG2a Recombinant Antibody (65599-1-MR, Clone:L17F12) (left) or 0.25 ug Anti-Human CD5 (65200-1-Ig, Clone: L17F12) (right) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65599-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

65599-1-PBS bindet in FC CD5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Humane T-akute lymphatische Leukämie (T-ALL) -Zellen
• Vollständiger Name: CD5 molecule
• Berechnetes Molekulargewicht: 495 aa, 55 kDa
• GenBank-Zugangsnummer: BC027901
• Gene symbol: CD5
• Gene ID (NCBI): 921
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).