CD53 Rekombinanter Antikörper

CD53 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242038C5

Kat-Nr. : 84837-1-RR

Synonyme

242038C5, Cell surface glycoprotein CD53, Leukocyte surface antigen CD53, MOX44, Tetraspanin 25

---

• 

  WB analysis using 84837-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84837-1-RR (CD53 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 84837-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84837-1-RR (CD53 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 84837-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84837-1-RR (CD53 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Daudi using 84837-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Daudi cells using CD53 antibody (84837-1-RR, Clone: 242038C5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of Jurkat using 84837-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD53 antibody (84837-1-RR, Clone: 242038C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 84837-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84837-1-RR against Human CD53 were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Jurkat-Zellen, Daudi-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84837-1-RR bindet in WB, IHC, IF/ICC, ELISA CD53 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD53 fusion protein Eg2182
• Vollständiger Name: CD53 molecule
• Berechnetes Molekulargewicht: 24kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: NM_000560.4
• Gene symbol: CD53
• Gene ID (NCBI): 963
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD53 is also named as MOX44 and Tetraspanin-25 (Tspan-25). Tetraspanin CD53 is a member of the tetraspanin superfamily expressed exclusively within the immune compartment (PMID: 32440787). T cells depend on the phosphatase CD45 to initiate T cell receptor signaling. CD53 is shown to stabilize CD45 on the membrane and is required for optimal phosphatase activity and subsequent Lck activation. Together, CD53 as a regulator of CD45 activity required for T cell immunity (PMID: 35767951). CD53 is a marker for positively selected CD4+ CD8+ thymocytes (PMID: 11867561).