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CD53 Rekombinanter Antikörper

CD53 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250105D10

Kat-Nr. : 85838-3-PBS

Synonyme

Cell surface glycoprotein CD53, Leukocyte surface antigen CD53

Filter:
  • Western Blot (WB) analysis of RAW 264.7 cells using CD53 Recombinant antibody (85838-3-RR)

    WB analysis of RAW 264.7 using 85838-3-RR (same clone as 85838-3-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 85838-3-RR (CD53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85838-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD53 Recombinant antibody (85838-3-RR)

    IHC staining of mouse spleen using 85838-3-RR (same clone as 85838-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85838-3-RR (Cd53 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85838-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD53 Recombinant antibody (85838-3-RR)

    IHC staining of mouse spleen using 85838-3-RR (same clone as 85838-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85838-3-RR (Cd53 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85838-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A20 cells using CD53 Recombinant antibody (85838-3-RR)

    IF Staining of A20 using 85838-3-RR (same clone as 85838-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A20 cells using Cd53 antibody (85838-3-RR, Clone: 250105D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85838-3-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

85838-3-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CD53 und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CD53 fusion protein Eg2684
Vollständiger Name CD53 antigen
Berechnetes Molekulargewicht24 kDa
Beobachtetes Molekulargewicht35-40 kDa
GenBank-ZugangsnummerNM_007651.3
Gene symbol Cd53
Gene ID (NCBI) 12508
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD53 is also named as MOX44 and Tetraspanin-25 (Tspan-25). Tetraspanin CD53 is a member of the tetraspanin superfamily expressed exclusively within the immune compartment (PMID: 32440787). T cells depend on the phosphatase CD45 to initiate T cell receptor signaling. CD53 is shown to stabilize CD45 on the membrane and is required for optimal phosphatase activity and subsequent Lck activation. Together, CD53 as a regulator of CD45 activity required for T cell immunity (PMID: 35767951). CD53 is a marker for positively selected CD4+ CD8+ thymocytes (PMID: 11867561).