CD53 Rekombinanter Antikörper

CD53 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

250105D10

Kat-Nr. : 98436-3-PBS

Synonyme

Cell surface glycoprotein CD53, Leukocyte surface antigen CD53

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  FC experiment of mouse splenocytes using 98436-3-RR (same clone as 98436-3-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.125 ug Anti-Mouse Cd53 Rabbit RecAb (98436-3-RR, Clone:250105D10) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 488 Anti-Mouse CD4. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98436-3-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98436-3-RR (same clone as 98436-3-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.125 ug Anti-Mouse Cd53 Rabbit RecAb (98436-3-RR, Clone: 250105D10) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9)(blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98436-3-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

98436-3-PBS bindet in FC CD53 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD53 fusion protein Eg2684
• Vollständiger Name: CD53 antigen
• Berechnetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: NM_007651.3
• Gene symbol: Cd53
• Gene ID (NCBI): 12508
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

CD53 is also named as MOX44 and Tetraspanin-25 (Tspan-25). Tetraspanin CD53 is a member of the tetraspanin superfamily expressed exclusively within the immune compartment (PMID: 32440787). T cells depend on the phosphatase CD45 to initiate T cell receptor signaling. CD53 is shown to stabilize CD45 on the membrane and is required for optimal phosphatase activity and subsequent Lck activation. Together, CD53 as a regulator of CD45 activity required for T cell immunity (PMID: 35767951). CD53 is a marker for positively selected CD4+ CD8+ thymocytes (PMID: 11867561).