ICAM-1/CD54 Rekombinanter Antikörper

ICAM-1/CD54 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

15.2

Kat-Nr. : 65509-1-RR

Synonyme

CD54, ICAM-1, 15.2, BB2, ICAM 1

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  FC experiment of human PBMCs using 65509-1-RR

  1x10^6 human PBMCs were surface stained with 0.05 ug Anti-Human CD54 Rabbit Recombinant Antibody (65509-1-RR, Clone:15.2) or Rabbit IgG control Rabbit PolyAb, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using 65509-1-RR

  1x10^6 human PBMCs were surface stained with 0.05 ug Anti-Human CD54 Rabbit Recombinant Antibody (65509-1-RR, Clone:15.2) or Rabbit IgG control Rabbit PolyAb, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using 65509-1-RR

  1x10^6 human PBMCs were surface stained with 0.05 ug Anti-Human CD54 Rabbit Recombinant Antibody (65509-1-RR, Clone:15.2) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000 (left and red), or stained with 0.05 ug Anti-Human CD54 Mouse Monoclonal Antibody (Clone:15.2) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000 (center and green). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65509-1-RR bindet in FC ICAM-1/CD54 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rheumatoide Synovialzellen und humane Monozyten
• Vollständiger Name: intercellular adhesion molecule 1
• Berechnetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC015969
• Gene symbol: ICAM-1
• Gene ID (NCBI): 3383
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).