ICAM-1/CD54 Monoklonaler Antikörper

ICAM-1/CD54 Monoklonal Antikörper für FC, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

15.2

Kat-Nr. : 65075-1-PBS

Synonyme

CD54 (ICAM-1), CD54, ICAM-1, 15.2, BB2

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  Sandwich ELISA standard curve of MP50085-1

  Sandwich ELISA standard curve of MP50085-1, ICAM-1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 65075-1-PBS. Detection antibody: 68693-1-PBS. Standard: Eg0570. Range: 62.5-4000 pg/mL.

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  Sample test of MP50085-1

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. The mean ICAM-1/CD54 concentration was determined to be 2,139.3 pg/mL in unstimulated PBMC supernatant, 30,678.8 pg/mL in PHA stimulated PBMC supernatant.

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  Sample test of MP50085-1

  Saliva of seven individual healthy human donors was measured. The human ICAM-1/CD54 concentration of detected samples was determined to be 5,597.4 pg/mL with a range of 2,577.6 - 11,698.1 pg/mL.

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  Sample test of MP50085-1

  Urine of seven individual healthy human donors was measured. The human ICAM-1/CD54 concentration of detected samples was determined to be 1,031.9 pg/mL with a range of 49.7 - 3,299.7 pg/mL.

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  Sample test of MP50085-1

  Serum of sixteen individual healthy human donors was measured. The human ICAM-1/CD54 concentration of detected samples was determined to be 260.08 ng/mL with a range of 142.73 - 650.42 ng/mL.

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  FC experiment of human PBMCs using 65075-1-Ig (same clone as 65075-1-PBS)

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD54 (ICAM-1) (65075-1-Ig, Clone:15.2) and APC-Donkey anti-Mouse IgG at dilution 1:1000. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65075-1-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 65075-1-Ig (same clone as 65075-1-PBS)

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD54 (ICAM-1) (65075-1-Ig, Clone:15.2) and APC-Donkey anti-Mouse IgG at dilution 1:1000. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65075-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

65075-1-PBS bindet in FC, Sandwich ELISA, Indirect ELISA, Sample test ICAM-1/CD54 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Rheumatoide Synovialzellen und humane Monozyten
• Vollständiger Name: intercellular adhesion molecule 1
• Berechnetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC015969
• Gene symbol: ICAM-1
• Gene ID (NCBI): 3383
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).