CD55 Polyklonaler Antikörper

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• KD/KO Validated

CD55 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 26580-1-AP

Synonyme

Complement decay-accelerating factor, CR, CROM, DAF, TC

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  WB analysis of HeLa using 26580-1-AP

  WB result of CD55 antibody (26580-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD55 transfected HeLa cells.

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  WB analysis using 26580-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26580-1-AP (CD55 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 26580-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26580-1-AP (CD55 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 26580-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26580-1-AP (CD55 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 26580-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26580-1-AP (CD55 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 26580-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26580-1-AP (CD55 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 26580-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CD55 antibody (26580-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, humanes Plazenta-Gewebe, MKN-45-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:4000-1:16000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Produktinformation

26580-1-AP bindet in WB, IHC, IF/ICC, ELISA CD55 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD55 fusion protein Ag25026
• Vollständiger Name: CD55 molecule, decay accelerating factor for complement (Cromer blood group)
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 60-80 kDa
• GenBank-Zugangsnummer: BC001288
• Gene symbol: CD55
• Gene ID (NCBI): 1604
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD55, also known as DAF, is a glycosylphosphatidylinositol (GPI)-anchored surface glycoprotein that is widely distributed on blood, stroma, epithelial, and endothelial cells (PMID: 7517044; 29503741). It can also exist as a soluble form in plasma, urine, saliva, tears, and synovial fluids (PMID: 29503741). CD55 is a complement regulatory protein (PMID: 2469439; 7517044). It inhibits formation of the C3 convertases through binding to C3b and C4b. It also binds the alternate pathway convertase C3bBb, the classical pathway convertase and C4b2a to accelerate their decay (PMID: 17289551). CD55 also serves as a receptor for coxsackieviruses B1, B3, and B5 and several enteroviruses (PMID: 7538177; 7517044). The observed molecular weight of mature CD55 varies between 50 to 100 kDa depending on the cell type. Different sizes of CD55 might be caused by alternative splicing or different glycosylation patterns (PMID: 29503741).

Publikationen

Species	Application	Title
human	WB,IHC,IF	Mol Cancer; Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer; Authors - Rashmi Bharti
rat	WB	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
human	WB	Clin Exp Immunol; Therapeutic hyperthermia regulates complement C3 activation and suppresses tumor development through HSPA5/NFκB/CD55 pathway in nasopharyngeal carcinoma; Authors - Chengcong Chen
rat	WB	Phytomedicine; Total coumarin derivates from Hydrangea paniculata attenuate renal injuries in cationized-BSA induced membranous nephropathy by inhibiting complement activation and interleukin 10-mediated interstitial fibrosis.; Authors - Weida Wang
	WB	J Proteomics; Quantitative proteomic analysis of scleras in guinea pig exposed to wavelength defocus.; Authors - Yingying Wen
rat,human	IF	Exp Ther Med; Paeoniflorin-6'O-benzene sulfonate suppresses fibroblast-like synoviocytes proliferation and migration in rheumatoid arthritis through regulating GRK2-Gβγ interaction; Authors - Yuwen Zhang