CD55 Rekombinanter Antikörper

CD55 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2D2

Kat-Nr. : 82781-6-PBS

Synonyme

TC, DAF, CROM, CR, Complement decay-accelerating factor

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  WB analysis using 82781-6-RR (same clone as 82781-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82781-6-RR (CD55 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82781-6-PBS in a different storage buffer formulation.

• 

  WB analysis using 82781-6-RR (same clone as 82781-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82781-6-RR (CD55 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82781-6-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 82781-6-RR (same clone as 82781-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82781-6-RR (CD55 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82781-6-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 82781-6-RR (same clone as 82781-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82781-6-RR (CD55 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82781-6-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 82781-6-RR (same clone as 82781-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82781-6-RR (CD55 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82781-6-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 82781-6-RR (same clone as 82781-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82781-6-RR (CD55 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82781-6-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 82781-6-RR (same clone as 82781-6-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CD55 antibody (82781-6-RR, Clone: 2D2 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82781-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82781-6-RR

  Biolayer interferometry (BLl) kinetic assays of 82781-6-RR against Human CD55 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

82781-6-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CD55 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD55 fusion protein Ag25026
• Vollständiger Name: CD55 molecule, decay accelerating factor for complement (Cromer blood group)
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC001288
• Gene symbol: CD55
• Gene ID (NCBI): 1604
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD55, also known as DAF, is a glycosylphosphatidylinositol (GPI)-anchored surface glycoprotein that is widely distributed on blood, stroma, epithelial, and endothelial cells (PMID: 7517044; 29503741). It can also exist as a soluble form in plasma, urine, saliva, tears, and synovial fluids (PMID: 29503741). CD55 is a complement regulatory protein (PMID: 2469439; 7517044). It inhibits formation of the C3 convertases through binding to C3b and C4b. It also binds the alternate pathway convertase C3bBb, the classical pathway convertase and C4b2a to accelerate their decay (PMID: 17289551). CD55 also serves as a receptor for coxsackieviruses B1, B3, and B5 and several enteroviruses (PMID: 7538177; 7517044). The observed molecular weight of mature CD55 varies between 50 to 100 kDa depending on the cell type. Different sizes of CD55 might be caused by alternative splicing or different glycosylation patterns (PMID: 29503741).