CD55 Rekombinanter Antikörper

CD55 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

241808D11

Kat-Nr. : CL647-98228

Synonyme

241808D11, Cd55a, Complement decay-accelerating factor, GPI-anchored, Daf, Daf1

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  FC experiment of mouse splenocytes using CL647-98228

  1x10^6 mouse splenocytes were surface stained with CoraLite® Plus 488 Anti-Mouse CD45R (B220) (RA3-6B2) (CL488-65139, Clone: RA3-6B2), and 0.25 ug CoraLite® Plus 647 Anti-Mouse CD55 Rabbit RecAb (CL647-98228, Clone: 241808D11) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were not fixed.

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  FC experiment of mouse splenocytes using CL647-98228

  1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD55 Rabbit RecAb (CL647-98228, Clone: 241808D11)(red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-98228 bindet in FC CD55 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD55 fusion protein Eg2124
• Vollständiger Name: CD55 antigen
• Berechnetes Molekulargewicht: 43kDa
• GenBank-Zugangsnummer: NM_010016.3
• Gene symbol: CD55
• Gene ID (NCBI): 13136
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD55, also known as DAF, is a glycosylphosphatidylinositol (GPI)-anchored surface glycoprotein that is widely distributed on blood, stroma, epithelial, and endothelial cells (PMID: 7517044; 29503741). It can also exist as a soluble form in plasma, urine, saliva, tears, and synovial fluids (PMID: 29503741). CD55 is a complement regulatory protein (PMID: 2469439; 7517044). It inhibits formation of the C3 convertases through binding to C3b and C4b. It also binds the alternate pathway convertase C3bBb, the classical pathway convertase and C4b2a to accelerate their decay (PMID: 17289551). CD55 also serves as a receptor for coxsackieviruses B1, B3, and B5 and several enteroviruses (PMID: 7538177; 7517044).