CD58 Polyklonaler Antikörper

CD58 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 10878-1-AP

Synonyme

Surface glycoprotein LFA 3, Lymphocyte function-associated antigen 3, LFA3, LFA 3, CD58 molecule

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  WB analysis using 10878-1-AP

  Various cells were subjected to SDS PAGE followed by western blot with 10878-1-AP (CD58 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of TF-1 using 10878-1-AP

  TF-1 cells were subjected to SDS PAGE followed by western blot with 10878-1-AP (CD58 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10878-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10878-1-AP (CD58 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human lymph using 10878-1-AP

  human lymph tissue were subjected to SDS PAGE followed by western blot with 10878-1-AP (CD58 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 10878-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10878-1-AP (CD58 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 10878-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10878-1-AP (CD58 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 10878-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 10878-1-AP (CD58 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, HeLa-Zellen, humanes Lymphgewebe, Jurkat-Zellen, TF-1-Zellen
• Erfolgreiche Detektion in IHC: human intrahepatic cholangiocarcinoma tissue, humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

10878-1-AP bindet in WB, IHC, ELISA CD58 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD58 fusion protein Ag1165
• Vollständiger Name: CD58 molecule
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 55-70 kDa
• GenBank-Zugangsnummer: BC005930
• Gene symbol: CD58
• Gene ID (NCBI): 965
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD58 a heavily glycosylated protein of 55-70 kDa, is expressed on a broad range of hematopoietic and non-hematopoietic cells. CD58 is involved in immune recognition of tumor cells via binding of the CD2 receptor expressed on cytotoxic T cells. The protein is localized to the plasma membrane.

Publikationen

Species	Application	Title
human	WB	Heliyon; Clinical significance of immune-related antigen CD58 in gliomas and analysis of its potential core related gene clusters; Authors - Zhi Tian