CD58 Rekombinanter Antikörper

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• KD/KO Validated

CD58 Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250250H5

Kat-Nr. : 85950-1-PBS

Synonyme

Ag3, CD58 molecule, LFA 3, LFA3, Lymphocyte function-associated antigen 3

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  WB analysis of LNCaP using 85950-1-RR (same clone as 85950-1-PBS)

  WB result of CD58 antibody (85950-1-RR; 1:1000; room temperature for 1.5 hours) with wild-type and CD58 knockout LNCaP cells. This data was developed using the same antibody clone with 85950-1-PBS in a different storage buffer formulation.

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  WB analysis using 85950-1-RR (same clone as 85950-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85950-1-RR (CD58 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85950-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 85950-1-RR (same clone as 85950-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85950-1-RR (CD58 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85950-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array sample test of MP02208-1

  The mean CD58 concentration was determined to be 126.7 ng/mL in human serum.

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  Cytometric bead array standard curve of MP02208-1

  Cytometric bead array standard curve of MP02208-1, CD58 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85950-1-PBS. Detection antibody: 85950-2-PBS. Standard: Eg4236. Range: 0.313-40 ng/mL.

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Geprüfte Anwendungen

Produktinformation

85950-1-PBS bindet in WB, Cytometric bead array, Indirect ELISA CD58 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD58 fusion protein Eg4236
• Vollständiger Name: CD58 molecule
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 55-70 kDa
• GenBank-Zugangsnummer: BC005930
• Gene symbol: CD58
• Gene ID (NCBI): 965
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a heavily glycosylated protein of 55-70 kDa that is expressed on a broad range of hematopoietic and non-hematopoietic cells. CD58 is involved in immune recognition of tumor cells via binding of the CD2 receptor expressed on cytotoxic T cells.