CD59 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CD59 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Rezensionen (1)

Kat-Nr. : 10742-1-AP

Synonyme

16.3A5, 1F5, 1F5 antigen, CD59, CD59 glycoprotein, EJ16, EJ30, EL32, G344, HRF 20, HRF20, MAC inhibitory protein, MAC IP, MACIF, MEM43, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18 20, Protectin

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Galerie der Validierungsdaten

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  WB analysis using 10742-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10742-1-AP (CD59 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 10742-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10742-1-AP (CD59 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10742-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10742-1-AP (CD59 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10742-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10742-1-AP (CD59 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 10742-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 10742-1-AP (CD59 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 10742-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10742-1-AP (CD59 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 10742-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10742-1-AP (CD59 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10742-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10742-1-AP (CD59 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10742-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10742-1-AP (CD59 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 10742-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10742-1-AP (CD59 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of BxPC-3 using 10742-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 10742-1-AP (CD59 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 10742-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human CD59 (10742-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, HeLa-Zellen, humanes Hirngewebe, HUVEC-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: BxPC-3-Zellen
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

10742-1-AP bindet in WB, IHC, IF, FC, ELISA CD59 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD59 fusion protein Ag1173
• Vollständiger Name: CD59 molecule, complement regulatory protein
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 18-20 kDa
• GenBank-Zugangsnummer: BC001506
• Gene symbol: CD59
• Gene ID (NCBI): 966
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.

Publikationen

Species	Application	Title
human	WB	Int J Mol Sci; Comparison of Fucoidans from Saccharina latissima Regarding Age-Related Macular Degeneration Relevant Pathomechanisms in Retinal Pigment Epithelium; Authors - Philipp Dörschmann
human	WB,IHC	Int J Oncol; The membrane complement regulatory protein CD59 promotes tumor growth and predicts poor prognosis in breast cancer.; Authors - Qianwen Ouyang
human	WB	PLoS One; Gecko CD59 is implicated in proximodistal identity during tail regeneration.; Authors - Wang Yongjun Y
	WB	Chem Res Toxicol; 4-Hydroxy-7-oxo-5-heptenoic Acid Lactone Is a Potent Inducer of the Complement Pathway in Human Retinal Pigmented Epithelial Cells.; Authors - Mikhail Linetsky
human	WB	Biochem Biophys Rep; Complement-dependent and -independent aquaporin 4-antibody-mediated cytotoxicity in human astrocytes: Pathogenetic implications in neuromyelitis optica.; Authors - S Nishiyama
human	WB,IF	Mol Cell Endocrinol; Endovascular trophoblast expresses CD59 to evade complement-dependent cytotoxicity.; Authors - Masashi Ueda ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Macarena Lucia (Verified Customer) (10-17-2022)

clear band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C