CD59 Monoklonaler Antikörper

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CD59 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

1E3A1

Kat-Nr. : 68222-1-PBS

Synonyme

1E3A1, 20 kDa homologous restriction factor, CD 59, CD59 antigen, CD59 glycoprotein

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  WB analysis using 68222-1-Ig (same clone as 68222-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68222-1-Ig (CD59 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68222-1-Ig (same clone as 68222-1-PBS)

  WB result of CD59 antibody (68222-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD59 transfected HeLa cells. This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  WB analysis using 68222-1-Ig (same clone as 68222-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68222-1-Ig (CD59 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IF Staining of BxPC-3 using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed BxPC-3 cells using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68222-1-PBS bindet in WB, IHC, IF/ICC, IF-P, ELISA CD59 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD59 fusion protein Ag32834
• Vollständiger Name: CD59 molecule, complement regulatory protein
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 18-25 kDa
• GenBank-Zugangsnummer: BC001506
• Gene symbol: CD59
• Gene ID (NCBI): 966
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.