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CD59 Rekombinanter Antikörper

CD59 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241310G8

Kat-Nr. : 84447-1-PBS

Synonyme

20 kDa homologous restriction factor, 241310G8, CD 59, CD59 antigen, CD59 glycoprotein

Filter:
  • Western Blot (WB) analysis of various lysates using CD59 Recombinant antibody (84447-1-RR)

    WB analysis using 84447-1-RR (same clone as 84447-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84447-1-RR (CD59 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84447-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CD59 Recombinant antibody (84447-1-RR)

    WB analysis of HeLa using 84447-1-RR (same clone as 84447-1-PBS)

    WB result of CD59 antibody (84447-1-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD59 transfected HeLa cells. This data was developed using the same antibody clone with 84447-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using CD59 Recombinant antibody (84447-1-RR)

    IF Staining of BxPC-3 using 84447-1-RR (same clone as 84447-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed BxPC-3 cells using CD59 antibody (84447-1-RR, Clone: 241310G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84447-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84447-1-RR

    Affinity and Kinetic Characterization of 84447-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84447-1-RR against Human CD59 were performed. The affinity constant is 1.59 nM.

Geprüfte Anwendungen

Produktinformation

84447-1-PBS bindet in WB, IF/ICC, Indirect ELISA CD59 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Rekombinantes Protein
Vollständiger Name CD59 molecule, complement regulatory protein
Berechnetes Molekulargewicht 19 kDa
Beobachtetes Molekulargewicht18-20 kDa
GenBank-ZugangsnummerBC001506
Gene symbol CD59
Gene ID (NCBI) 966
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.