CD59 Rekombinanter Antikörper

CD59 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

FITC Plus Fluorescent Dye

CloneNo.

241311A4

Kat-Nr. : FITC-98099

Synonyme

20 kDa homologous restriction factor, 241311A4, CD 59, CD59 antigen, CD59 glycoprotein

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  FC experiment of human PBMCs using FITC-98099

  1x10^6 human PBMCs were surface stained with APC Anti-Human CD3 (OKT3), and 5 ul FITC Plus Anti-Human CD59 Rabbit RecAb (FITC-98099, Clone: 241311A4) or FITC Plus Rabbit IgG Isotype Control Recombinant Antibody (FITC-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using FITC-98099

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD59 Rabbit RecAb (FITC-98099, Clone: 241311A4) (red) or FITC Plus Rabbit IgG Isotype Control Recombinant Antibody (FITC-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-98099 bindet in FC CD59 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: CD59 molecule, complement regulatory protein
• Berechnetes Molekulargewicht: 19 kDa
• GenBank-Zugangsnummer: BC001506
• Gene symbol: CD59
• Gene ID (NCBI): 966
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.