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CD5L Polyklonaler Antikörper

CD5L Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17224-1-PBS

Synonyme

hAIM, CT-2, CD5 antigen-like, Apoptosis inhibitor expressed by macrophages, API6

Filter:
  • Western Blot (WB) analysis of human plasma using CD5L Polyclonal antibody (17224-1-AP)

    WB analysis of human plasma using 17224-1-AP (same clone as 17224-1-PBS)

    human plasma was subjected to SDS PAGE followed by western blot with 17224-1-AP (CD5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17224-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using CD5L Polyclonal antibody (17224-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 17224-1-AP (same clone as 17224-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 17224-1-AP (CD5L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17224-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CD5L Polyclonal antibody (17224-1-AP)

    IF Staining of HepG2 using 17224-1-AP (same clone as 17224-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CD5L antibody (17224-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17224-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

17224-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CD5L und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CD5L fusion protein Ag11062
Vollständiger Name CD5 molecule-like
Berechnetes Molekulargewicht 347 aa, 38 kDa
Beobachtetes Molekulargewicht40 kDa
GenBank-ZugangsnummerBC033586
Gene symbol CD5L
Gene ID (NCBI) 922
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD5 molecule-like (CD5L), also known as apoptosis inhibitor expressed by macrophages (AIM), SP-alpha, apoptosis inhibitor 6 (API6), is a member of the scavenger receptor cysteine-rich (SRCR) family of proteins (PMID: 9045627; 9892623). CD5L is a soluble protein mainly secreted mostly by macrophages in lymphoid and inflamed tissues (PMID: 15009805). It contains three SRCR domains, each approximately 100 amino acids in length with sialic acid content at a potential region of O-linked glycosylation between SRCR domains 1 and 2. CD5L is involved in different processes such as metabolism, infection and autophagy (PMID: 33920819).