CD61 Monoklonaler Antikörper

CD61 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / Mouse IgG1 kappa

Getestete Reaktivität

human, nicht-humane Primaten

Anwendung

IF, FC

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

VIPL2

Kat-Nr. : 65185-1-Ig

Synonyme

CD61, GP3A, GPIIIa, Integrin beta 3, ITGB3

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Galerie der Validierungsdaten

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  FC experiment of human peripheral blood platelets using 65185-1-Ig

  Human peripheral blood platelets were surface stained with Purified Anti-Human CD61 (65185-1-Ig, Clone:VIPL2) (red) or Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Human peripheral blood platelets

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

65185-1-Ig bindet in IF, FC CD61 und zeigt Reaktivität mit human, nicht-humane Primaten

• Getestete Reaktivität: human, nicht-humane Primaten
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / Mouse IgG1 kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
• Berechnetes Molekulargewicht: 788 aa, 87 kDa
• GenBank-Zugangsnummer: BC127666
• Gene symbol: ITGB3
• Gene ID (NCBI): 3690
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Mit Protein-A-Affinitätschromatographie gereinigt
• Lagerungspuffer: PBS mit 0,1% Natriumazid.
• Lagerungsbedingungen: Bei 2-8℃ lagern.

Hintergrundinformationen

CD61 is a 110-kDa membrane glycoprotein that is also termed integrin β3 chain or GPIIIa. CD61 can associate with integrin αV chain (CD51) or integrin αIIb chain (CD41, GPIIb) to form heterodimeric receptors for cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, plasminogen, prothrombin, thrombospondin, vitronectin and von Willebrand factor (PMID: 7694604; 9251239; 2018971; 20628538). Integrin αVβ3 (CD51-CD61) is present on endothelium, smooth muscle, osteoclasts, platelets, some fibroblasts, some activated leukocytes and tumor cells (PMID: 7694604). GPllb-llla (CD41-CD61) is restricted in distribution to platelets and cells of megakaryoblastic potential and is required for platelet aggregation (PMID: 2018971; 16322781).

Publikationen

Species	Application	Title
human	IF	Small Methods; Superresolution Fluorescence Microscopy of Platelet Subcellular Structures as a Potential Tumor Liquid Biopsy; Authors - Peng Xu