CD62L Rekombinanter Antikörper

CD62L Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

250271C5

Kat-Nr. : 98441-3-PBS

Synonyme

L-selectin, CD62 antigen-like family member L, L selectin, LAM-1, LECAM 1

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  FC experiment of mouse splenocytes using 98441-3-RR (same clone as 98441-3-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD62L Rabbit RecAb (98441-3-RR, Clone:250271C5) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98441-3-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98441-3-RR (same clone as 98441-3-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD62L Rabbit RecAb (98441-3-RR, Clone:250271C5) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98441-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98441-3-RR

  Biolayer interferometry (BLl) kinetic assays of 98441-3-RR against Mouse CD62L were performed. The affinity constant is 1.00 nM.

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Geprüfte Anwendungen

Produktinformation

98441-3-PBS bindet in FC CD62L und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD62L fusion protein Eg3845
• Vollständiger Name: selectin, lymphocyte
• Berechnetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: NM_011346.2
• Gene symbol: CD62L
• Gene ID (NCBI): 20343
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

CD62L, also known as L-selectin or SELL, is a member of the selectin family of adhesion molecules that also include CD62E (E-selectin) and CD62P (P-selectin) (PMID: 2663882, 2473156, 1382078). CD62L is a highly glycosylated protein of 95-105 kDa on neutrophils and 74 kDa on lymphocytes (PMID: 1382078; 1694883, 1695155). CD62L is expressed on the surface of most leukocytes, including lymphocytes, neutrophils, monocytes, eosinophils, hematopoietic progenitor cells, and immature thymocytes (PMID: 1694883, 1688580). It mediates the binding of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes through interactions with a constitutively expressed ligand, and is also involved in lymphocyte, neutrophil, and monocyte attachment to endothelium at sites of inflammation (PMID: 1382078).