CD63 Polyklonaler Antikörper

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• KD/KO Validated

CD63 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25682-1-PBS

Synonyme

CD 63, CD63 antigen, Limp1, Lysosomal-associated membrane protein 3, Lysosome integral membrane protein 1

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  WB analysis of human urine exosomes using 25682-1-AP (same clone as 25682-1-PBS)

  human urine exosomes tissue were subjected to SDS PAGE followed by western blot with 25682-1-AP (CD63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 25682-1-AP (same clone as 25682-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25682-1-AP (CD63 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

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  WB analysis of human serum exosomes using 25682-1-AP (same clone as 25682-1-PBS)

  Human serum exosomes were subjected to SDS PAGE followed by western blot with 25682-1-AP (CD63 antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

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  WB analysis using 25682-1-AP (same clone as 25682-1-PBS)

  Human plasma and human plasma-derived exosomes (human plasma-exo) were subjected to SDS PAGE followed by western blot with 25682-1-AP (CD63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

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  IHC staining of human malignant melanoma using 25682-1-AP (same clone as 25682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

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  IHC staining of human malignant melanoma using 25682-1-AP (same clone as 25682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 25682-1-AP (same clone as 25682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 25682-1-AP (same clone as 25682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human malignant melanoma using 25682-1-AP (same clone as 25682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 10x lens). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human malignant melanoma using 25682-1-AP (same clone as 25682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 40x lens). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 25682-1-AP (same clone as 25682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 25682-1-AP (same clone as 25682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 25682-1-AP (same clone as 25682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 25682-1-AP (same clone as 25682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 25682-1-AP (same clone as 25682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 25682-1-AP (same clone as 25682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human malignant melanoma using 25682-1-AP (same clone as 25682-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human malignant melanoma tissue using CD63 antibody (25682-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human malignant melanoma using 25682-1-AP (same clone as 25682-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human malignant melanoma tissue using CD63 antibody (25682-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 25682-1-AP (same clone as 25682-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CD63 antibody (25682-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

25682-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA CD63 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD63 fusion protein Ag19690
• Vollständiger Name: CD63 molecule
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 30-60 kDa
• GenBank-Zugangsnummer: BC002349
• Gene symbol: CD63
• Gene ID (NCBI): 967
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediates signal transduction events that play a role in the regulation of cell development, activation, growth, and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.