CD63 Monoklonaler Antikörper

CD63 Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3D4D1

Kat-Nr. : 67605-1-Ig

Synonyme

CD63, CD63 antigen, CD63 molecule, Granulophysin, ME491, MLA1, OMA81H, Tetraspanin 30, Tspan 30, TSPAN30



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, A549-Zellen, HUVEC-Zellen, K-562-Zellen, LNCaP-Zellen, MCF-7-Zellen, THP-1-Zellen, U2OS-Zellen
Erfolgreiche Detektion in IHChumanes Tonsillitisgewebe, humanes Kolonkarzinomgewebe, humanes malignes Melanomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFhumanes Tonsillitisgewebe, humanes Lymphomgewebe, humanes malignes Melanomgewebe
Erfolgreiche Detektion in FCTHP-1-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:10000
Immunhistochemie (IHC)IHC : 1:350-1:1400
Immunfluoreszenz (IF)IF : 1:200-1:800
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

67605-1-Ig bindet in WB, IHC, IF, FC, ELISA CD63 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Ratte, Ziege
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen CD63 fusion protein Ag19690
Vollständiger Name CD63 molecule
Berechnetes Molekulargewicht 26 kDa
Beobachtetes Molekulargewicht 35 kDa
GenBank-ZugangsnummerBC002349
Gene symbol CD63
Gene ID (NCBI) 967
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediate signal transduction events that play a role in the regulation of cell development, activation and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.

Protokolle

Produktspezifische Protokolle
WB protocol for CD63 antibody 67605-1-IgProtokoll herunterladen
IHC protocol for CD63 antibody 67605-1-IgProtokoll herunterladen
IF protocol for CD63 antibody 67605-1-IgProtokoll herunterladen
FC protocol for CD63 antibody 67605-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Drug Des Devel Ther

Keloid Patient Plasma-Derived Exosomal hsa_circ_0020792 Promotes Normal Skin Fibroblasts Proliferation, Migration, and Fibrogenesis via Modulating miR-193a-5p and Activating TGF-β1/Smad2/3 Signaling

Authors - Huan Hu
WB

Oxid Med Cell Longev

Adipose-Derived Circulating Exosomes Promote Protection of the Pulmonary Endothelial Barrier by Inhibiting EndMT and Oxidative Stress through Down-Regulation of the TGF-β Pathway: A Potential Explanation for the Obesity Paradox in ARDS.

Authors - Di Qi
humanWB

Aging (Albany NY)

MSC-derived exosomes promote recovery from traumatic brain injury via microglia/macrophages in rat.

Authors - Yunfei Chen
humanWB

Aging (Albany NY)

Exosomal circRNAs contribute to intestinal development via the VEGF signalling pathway in human term and preterm colostrum.

Authors - Yahui Zhou
WB

Aging (Albany NY)

Endothelial cell-derived exosomal circHIPK3 promotes the proliferation of vascular smooth muscle cells induced by high glucose via the miR-106a-5p/Foxo1/Vcam1 pathway.

Authors - Shaohua Wang
WB

Int J Mol Sci

MicroRNAs in Small Extracellular Vesicles from Amniotic Fluid and Maternal Plasma Associated with Fetal Palate Development in Mice

Authors - Xige Zhao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Guorong (Verified Customer) (03-22-2022)

Two bands at 30 and 60 kDa was detected

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: ARPE-19
CD63 Antibody Western Blot validation (1:5000 dilution) in ARPE-19 (Cat no:67605-1-Ig)