CD63 Monoklonaler Antikörper

CD63 Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

3D4D1

Kat-Nr. : 67605-1-PBS

Synonyme

3D4D1, CD 63, CD63 antigen, Limp1, Lysosomal-associated membrane protein 3

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  WB analysis using 67605-1-Ig (same clone as 67605-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67605-1-Ig (CD63 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

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  WB analysis using 67605-1-Ig (same clone as 67605-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67605-1-Ig (CD63 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:700 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:700 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

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  IHC staining of human malignant melanoma using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

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  IHC staining of human malignant melanoma using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

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  IF Staining of human lymphoma using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human lymphoma using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human malignant melanoma using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human malignant melanoma tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human malignant melanoma using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human malignant melanoma tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67605-1-PBS bindet in WB, IHC, IF-P, ELISA CD63 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD63 fusion protein Ag19690
• Vollständiger Name: CD63 molecule
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC002349
• Gene symbol: CD63
• Gene ID (NCBI): 967
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediate signal transduction events that play a role in the regulation of cell development, activation and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.