CD63 Rekombinanter Antikörper

CD63 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241964H1

Kat-Nr. : 84507-4-PBS

Synonyme

Lysosome integral membrane protein 1, Lysosomal-associated membrane protein 3, Limp1, CD63 antigen, CD 63

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  WB analysis using 84507-4-RR (same clone as 84507-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84507-4-RR (CD63 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84507-4-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84507-4-RR (same clone as 84507-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84507-4-RR (CD63 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84507-4-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84507-4-RR (same clone as 84507-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84507-4-RR (CD63 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84507-4-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 84507-4-RR (same clone as 84507-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CD63 antibody (84507-4-RR, Clone: 241964H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84507-4-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84507-4-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CD63 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FusionProtein
• Vollständiger Name: CD63 molecule
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 30-60 kDa
• GenBank-Zugangsnummer: BC002349
• Gene symbol: CD63
• Gene ID (NCBI): 967
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediates signal transduction events that regulate cell development, activation, growth, and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein translocated to the plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it is a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.