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CD64 Polyklonaler Antikörper

CD64 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27563-1-PBS

Synonyme

CD64A, Fc gamma RI, Fc gamma RIA, FCG1, Fc-gamma RI

Filter:
  • Western Blot (WB) analysis of various lysates using CD64 Polyclonal antibody (27563-1-AP)

    WB analysis using 27563-1-AP (same clone as 27563-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 27563-1-AP (CD64 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27563-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using CD64 Polyclonal antibody (27563-1-AP)

    WB analysis of U-937 using 27563-1-AP (same clone as 27563-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 27563-1-AP (CD64 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27563-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using CD64 Polyclonal antibody (27563-1-AP)

    WB analysis of HL-60 using 27563-1-AP (same clone as 27563-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 27563-1-AP (CD64 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27563-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using CD64 Polyclonal antibody (27563-1-AP)

    IF Staining of THP-1 using 27563-1-AP (same clone as 27563-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD64 antibody (27563-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 27563-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

27563-1-PBS bindet in WB, IF/ICC, Indirect ELISA CD64 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CD64 fusion protein Ag26727
Vollständiger Name Fc fragment of IgG, high affinity Ia, receptor (CD64)
Berechnetes Molekulargewicht 374 aa, 43 kDa
Beobachtetes Molekulargewicht 65-70 kDa, 43-45 kDa
GenBank-ZugangsnummerBC032634
Gene symbol FCGR1A
Gene ID (NCBI) 2209
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses. The calculated molecular weight of CD64 is 43 kDa, while the glycosylated CD64 has a higher apparent molecular weight.