CD64 Polyklonaler Antikörper

CD64 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32060-1-AP

Synonyme

Fcg1, Fc-gamma RI, Fcgr1, FcRI, High affinity immunoglobulin gamma Fc receptor I

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  WB analysis using 32060-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32060-1-AP (CD64 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 32060-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 32060-1-AP (CD64 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 32060-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 32060-1-AP (CD64 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 32060-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32060-1-AP (CD64 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 32060-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32060-1-AP (CD64 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: J774A.1 cells, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: Mausmilzgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

32060-1-AP bindet in WB, IHC, ELISA CD64 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD64 fusion protein Eg2107
• Vollständiger Name: Fc receptor, IgG, high affinity I
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: NM_010186.5
• Gene symbol: Fcgr1
• Gene ID (NCBI): 14129
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses. The calculated molecular weight of CD64 is 43 kDa, while the glycosylated CD64 has a higher apparent molecular weight.