CD64 Rekombinanter Antikörper

CD64 Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

10.1

Kat-Nr. : 65572-1-MR

Synonyme

CD64, 10.1, CD64A, Fc gamma RI, Fc gamma RIA

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  FC experiment of human PBMCs using 65572-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD64 Mouse IgG2a Recombinant Antibody (65572-1-MR, Clone: 10.1) or 0.25 ug Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 65572-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD64 Mouse IgG2a Recombinant Antibody (65572-1-MR, Clone: 10.1) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 65572-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD64 Mouse IgG2a Recombinant Antibody (65572-1-MR, Clone: 10.1) (left) or 0.25 ug Anti-Human CD64 (65253-1-Ig, Clone: 10.1) (right) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65572-1-MR bindet in FC CD64 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.
• Vollständiger Name: Fc fragment of IgG, high affinity Ia, receptor (CD64)
• Berechnetes Molekulargewicht: 374 aa, 43 kDa
• GenBank-Zugangsnummer: BC032634
• Gene symbol: FCGR1A
• Gene ID (NCBI): 2209
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high-affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses.