CD64 Rekombinanter Antikörper

CD64 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241809D6

Kat-Nr. : 84518-2-RR

Synonyme

High affinity immunoglobulin gamma Fc receptor I, FcRI, Fcgr1, Fc-gamma RI, Fcg1

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  WB analysis using 84518-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84518-2-RR (CD64 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of RAW 264.7 using 84518-2-RR

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD64/FCGR1 antibody (84518-2-RR, Clone: 241809D6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84518-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84518-2-RR against Mouse CD64 were performed. The affinity constant is 74.4 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen, J774A.1 cells
• Erfolgreiche Detektion in IF/ICC: RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84518-2-RR bindet in WB, IF/ICC, ELISA CD64 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: Fc receptor, IgG, high affinity I
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 68-72 kDa
• GenBank-Zugangsnummer: NM_010186.5
• Gene symbol: Fcgr1
• Gene ID (NCBI): 14129
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high-affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses.