CD64 Rekombinanter Antikörper

CD64 Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Biotin

CloneNo.

10.1

Kat-Nr. : Biotin-65572

Synonyme

Fc-gamma RI, FCG1, Fc gamma RIA, Fc gamma RI, CD64A

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  FC experiment of human PBMCs using Biotin-65572

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD64 (10.1) Mouse IgG2a RecAb (Biotin-65572, Clone:10.1) and CoraLite®488-conjugated streptavidin, or unstained. Cells were co-stained with APC Anti-Human CD14 Rabbit Recombinant Antibody (APC-98040, Clone: 230332D7). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using Biotin-65572

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD64 (10.1) Mouse IgG2a RecAb (Biotin-65572, Clone:10.1) and CoraLite®488-conjugated streptavidin (red) or unstained (blue).Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

Biotin-65572 bindet in FC CD64 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.
• Vollständiger Name: Fc fragment of IgG, high affinity Ia, receptor (CD64)
• Berechnetes Molekulargewicht: 374 aa, 43 kDa
• GenBank-Zugangsnummer: BC032634
• Gene symbol: FCGR1A
• Gene ID (NCBI): 2209
• Konjugation: Biotin
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high-affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses.