CD64 Rekombinanter Antikörper

CD64 Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite®700 Fluorescent Dye

CloneNo.

10.1

Kat-Nr. : CL700-65572

Synonyme

CD64, 10.1, CD64A, Fc gamma RI, Fc gamma RIA

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  FC experiment of human PBMCs using CL700-65572

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD14, and 5 ul CoraLite®700 Anti-Human CD64 (10.1) Mouse IgG2a RecAb (CL700-65572, Clone:10.1) or unstained. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using CL700-65572

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite®700 Anti-Human CD64 (10.1) Mouse IgG2a RecAb (CL700-65572, Clone:10.1) (red) or unstained (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL700-65572 bindet in FC CD64 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.
• Vollständiger Name: Fc fragment of IgG, high affinity Ia, receptor (CD64)
• Berechnetes Molekulargewicht: 374 aa, 43 kDa
• GenBank-Zugangsnummer: BC032634
• Gene symbol: FCGR1A
• Gene ID (NCBI): 2209
• Konjugation: CoraLite®700 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 702 nm / 723 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high-affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses.