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CD64 Rekombinanter Antikörper

CD64 Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241809D6

Kat-Nr. : 84518-2-PBS

Synonyme

High affinity immunoglobulin gamma Fc receptor I, FcRI, Fcgr1, Fc-gamma RI, Fcg1

Filter:
  • Western Blot (WB) analysis of various lysates using CD64 Recombinant antibody (84518-2-RR)

    WB analysis using 84518-2-RR (same clone as 84518-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84518-2-RR (CD64 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84518-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CD64 Recombinant antibody (84518-2-RR)

    IF Staining of RAW 264.7 using 84518-2-RR (same clone as 84518-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD64/FCGR1 antibody (84518-2-RR, Clone: 241809D6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84518-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01366-1

    Cytometric bead array standard curve of MP01366-1

    Cytometric bead array standard curve of MP01366-1, MOUSE CD64 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84518-2-PBS. Detection antibody: 84518-1-PBS. Standard: Eg2107. Range: 0.625-40 ng/mL.

  • Affinity and Kinetic Characterization of 84518-2-RR

    Affinity and Kinetic Characterization of 84518-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84518-2-RR against Mouse CD64 were performed. The affinity constant is 74.4 pM.

Geprüfte Anwendungen

Produktinformation

84518-2-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA CD64 und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Rekombinantes Protein
Vollständiger Name Fc receptor, IgG, high affinity I
Berechnetes Molekulargewicht45 kDa
Beobachtetes Molekulargewicht68-72 kDa
GenBank-ZugangsnummerNM_010186.5
Gene symbol Fcgr1
Gene ID (NCBI) 14129
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high-affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses.