CD68 Polyklonaler Antikörper

CD68 Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30929-1-PBS

Synonyme

CD68 antigen, gp110, Scard1

---

• 

  WB analysis using 30929-1-AP (same clone as 30929-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30929-1-AP (CD68 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 30929-1-AP (same clone as 30929-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 30929-1-AP (same clone as 30929-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 30929-1-AP (same clone as 30929-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 30929-1-AP (same clone as 30929-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 30929-1-AP (same clone as 30929-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 30929-1-AP (same clone as 30929-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 30929-1-AP (same clone as 30929-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 30929-1-AP (same clone as 30929-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 30929-1-AP (same clone as 30929-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (30929-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 30929-1-AP (same clone as 30929-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (30929-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

30929-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA CD68 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD68 fusion protein Eg0787
• Vollständiger Name: CD68 antigen
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: NM_001291058.1
• Gene symbol: Cd68
• Gene ID (NCBI): 12514
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.