CD68 Monoklonaler Antikörper

CD68 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

KP1

Kat-Nr. : 65202-1-Ig

Synonyme

CD68, CD68 molecule, GP110, KP1, Macrosialin, SCARD1

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  FC experiment of human PBMCs using 65202-1-Ig

  1X10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD68 (65202-1-Ig, Clone:KP1) and FITC-Donkey anti-Mouse IgG at dilution 1:1000 (red), or Mouse IgG1 Isotype Control and FITC-Donkey anti-Mouse IgG at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

• 

  FC experiment of human PBMCs using 65202-1-Ig

  1X10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD68 (65202-1-Ig, Clone:KP1) and FITC-Donkey anti-Mouse IgG at dilution 1:1000, or Mouse IgG1 Isotype Control and FITC-Donkey anti-Mouse IgG at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): humane PBMCs
• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65202-1-Ig bindet in FC (Intra) CD68 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Lysosomal fraction of human alveolar macrophages
• Vollständiger Name: CD68 molecule
• Berechnetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC015557
• Gene symbol: CD68
• Gene ID (NCBI): 968
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.