CD68 Monoklonaler Antikörper

CD68 Monoklonal Antikörper für IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(86)

CloneNo.

3A9A7

Rezensionen (2)

Kat-Nr. : 66231-2-Ig

Synonyme

3A9A7, CD68 molecule, GP110, Macrosialin, SCARD1

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  IHC staining of human tonsillitis using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66231-2-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 66231-2-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD68 antibody (66231-2-Ig, Clone: 3A9A7 ) at dilution of 1:6000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human tonsillitis using 66231-2-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD68 antibody (66231-2-Ig, Clone: 3A9A7 ) at dilution of 1:6000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Appendizitis-Gewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Urothelkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:1000-1:6000
• Immunfluoreszenz (IF)-P: IF-P : 1:3000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 32 publications below
• IF: See 51 publications below

Produktinformation

66231-2-Ig bindet in IHC, IF-P, ELISA CD68 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD68 fusion protein Ag22815
• Vollständiger Name: CD68 molecule
• Berechnetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC015557
• Gene symbol: CD68
• Gene ID (NCBI): 968
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Publikationen

Species	Application	Title
human	IF	Cell Rep Med; Microneedle delivery of CAR-M-like engineered macrophages alleviates intervertebral disc degeneration through enhanced efferocytosis capacity; Authors - Xingyu Zhou
	IHC	J Clin Invest; Lactate inhibits ATP6V0d2 expression in tumor-associated macrophages to promote HIF-2α-mediated tumor progression.; Authors - Na Liu
	IF	Adv Healthc Mater; Wireless Electric Cues Mediate Autologous DPSC-Loaded Conductive Hydrogel Microspheres to Engineer The Immuno-Angiogenic Niche for Homologous Maxillofacial Bone Regeneration; Authors - Jiwei Sun
	IHC	Redox Biol; Redox-sensitive activation of CCL7 by BRG1 in hepatocytes during liver injury.; Authors - Ming Kong
		Redox Biol; Quercetin hinders microglial activation to alleviate neurotoxicity via the interplay between NLRP3 inflammasome and mitophagy.; Authors - Xiaojuan Han
human	IF	Transl Res; Purinergic receptor P2 × 7 contributes to abdominal aortic aneurysm development via modulating macrophage pyroptosis and inflammation; Authors - Likun Sun

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Cohen (Verified Customer) (06-02-2025)

Received a generous test sample of 20ul which stretched far. While the suggested dilutions ranged from 1/3000 to 12000, I found that 1/3000 was optimal for human adipose tissue and ectopic endometrial tissue. This is very impressive for a primary antibody, in my experience.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/3000
• Cell Tissue Type: Human adipose and ectopic endometrial tissue

FH

Balawant (Verified Customer) (12-25-2023)

This excellent antibody has no background.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: colon tissue